Journal:

Article Title: Localization of Sodium Channels in Intercalated Disks Modulates Cardiac Conduction

doi:

Figure Lengend Snippet: Double-labeling immunocytochemistry for NaChs and Cx43 in a culture of neonatal rat ventricular myocytes. A, NaChs. The increase in fluorescence along the boundaries between cells (arrows) indicates a local clustering of NaChs at the sites of cell-to-cell appositions. The concomitant increase in fluorescence in the region of the nuclei is most likely nonspecific (see text). B, Cx43. The subcellular distribution of Cx43 in the same preparation is highly correlated to that of NaChs suggesting a high degree of colocalization of the two channels as illustrated by superposition of the images (C).

Article Snippet: Primary antibodies, directed against rat heart NaChs (rH1, polyclonal antibody raised against residues 492 to 510 of the α-subunit of rat cardiac type NaCh; Alomone) and Cx43 (mouse anti-Cx43 monoclonal antibody; Chemicon) were diluted at 1:10 (rH1) and 1:100 (Cx43) in a buffer containing 1% goat serum and 0.15% Triton X-100.

Techniques: Labeling, Immunocytochemistry, Fluorescence